Tadalafil combined with remote ischemic preconditioning in the prevention of renal ischemia/reperfusion injury


  • Italo Medeiros Azevedo Federal University of Rio Grande do Norte (UFRN) https://orcid.org/0000-0002-1507-7401
  • Gabriela Vieira da Silva
  • Aristela Sinara Varela Constantino de Medeiros
  • Ariadne Sibelle Varela Constantino de Medeiros Dumaresq de Souza
  • Rosana Karla Aires de Macêdo
  • Aldo Cunha Medeiros




PURPOSE: This study aimed to evaluate whether the combination of tadalafil treatment and remote ischemic preconditioning may provide protection against ischemia reperfusion injury (I/R) in kidneys of rats. METHODS: Twenty-four male Wistar rats weighing 280-300g were randomly divided into 3 groups: Group 1 - (control), only right nephrectomy was performed. No treatment with tadalafil. Group II - Left renal ischemia, right nephrectomy, without remote preconditioning, treatment with tadalafil (1.0 mg/kg, oral). Group III - Left renal ischemia, right nephrectomy, with remote preconditioning, treatment with tadalafil.  RESULTS: 24 h after, urea and creatinine dosage and histopathology of the ischemic kidneys were performed. In the left kidney of control group rats, a higher degree of cellular necrosis was observed when compared to the tadalafil group, with and without remote preconditioning. On the preconditioning + tadalafil group rats, there was a significant reduction in histological parameters when compared to the Ischemia + tadalafil group rats (p<0.05). In group III, comparing to other groups, a significant improvement of the left kidney function was observed, through the lower values ??of the urea and creatinine dosages. CONCLUSION: This study demonstrated protective effects of remote preconditioning + tadalafil in an experimental model of renal I/R in the rat. The results demonstrated that a single dose of tadalafil prior to renal I/R attenuated the histopathological lesions, especially cellular necrosis, and renal function tests.


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How to Cite

AZEVEDO, I. M.; VIEIRA DA SILVA, G. .; SINARA VARELA CONSTANTINO DE MEDEIROS, A.; SIBELLE VARELA CONSTANTINO DE MEDEIROS DUMARESQ DE SOUZA, A.; KARLA AIRES DE MACÊDO, R.; CUNHA MEDEIROS, A. Tadalafil combined with remote ischemic preconditioning in the prevention of renal ischemia/reperfusion injury. JOURNAL OF SURGICAL AND CLINICAL RESEARCH, [S. l.], v. 9, n. 1, p. 11–23, 2018. DOI: 10.20398/jscr.v9i1.15731. Disponível em: https://periodicos.ufrn.br/jscr/article/view/15731. Acesso em: 6 oct. 2022.