Effect of hydration with Ringer's solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in rats

  • Juliana Mendonça Freire
  • Jeovana Pinheiro Fernandes de Souza
  • Italo Medeiros Azevedo Federal University of Rio Grande do Norte https://orcid.org/0000-0002-1507-7401
  • Fabio Medeiros de Azevedo Federal University of Rio Grande do Norte
  • Artur da Silva Carriço Federal University of Rio Grande do Norte
  • Aldo Cunha Medeiros Federal University of Rio Grande do Norte

Abstract

Purpose: To evaluate the effect of hydration with Ringer's solution submitted to magnetic field in experimental model of hepatic ischemia/reperfusion in rats. Methods: Wistar rats weighing 389.58±46.88g were randomly allocated into 2 groups: control group (n = 6) - Hepatic ischemia/reperfusion and hydration with Ringer's solution; and test group (n = 6) - Hepatic ischemia/reperfusion and hydration with Ringer submitted to magnetic field of 20 mT during 2 hours. After anesthesia with ketamine 70 mg/kg and xylazine 7 mg/kg i.p., the animals were weighed and operated by aseptic technique. The ischemia of the median and left liver lobes was induced for 30 minutes using a vascular clip. Then, i.v. hydration with 3 ml of Ringer in the control group, and 3 ml of magnetized Ringer’s solution in the test group. After 24 hours, blood sample and fragment of the left liver lobe were collected for the determination of AST, ALT, ALP and albumin levels, and histopathological examination. Results: There was no significant difference in comparison of the rats weight loss, biochemical measurements and histopathological scores between the groups (p>0.05). Conclusion: The hydration with Ringer's solution submitted to magnetic field after hepatic ischemia/reperfusion in rats did not alter liver function tests neither histopathological scores, comparing with the control group.

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Published
09-11-2018
How to Cite
FREIRE, J. M.; SOUZA, J. P. F. DE; AZEVEDO, I. M.; AZEVEDO, F. M. DE; CARRIÇO, A. DA S.; MEDEIROS, A. C. Effect of hydration with Ringer’s solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in rats. JOURNAL OF SURGICAL AND CLINICAL RESEARCH, v. 9, n. 2, p. 44-53, 9 Nov. 2018.
Section
ORIGINAL ARTICLE