EFFECT OF PNEUMOPERITONEUM IN MODEL OF ABDOMINAL SEPSIS

  • Cybelle Dutra Silva Medical student
  • Evelynne Mayara Araújo Silva Medical student
  • Vítor Brasil Medeiros Hospital Veterinário
  • Aldo Cunha Medeiros Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil https://orcid.org/0000-0003-2372-942X
Keywords: Sepsis. Pneumoperitoneum. Cytokines. Procalcitonin. C-reactive protein. Inflammation.

Abstract

ABSTRACT

Purpose: This study aims to examine the effects of CO2 pneumoperitoneum on the level of inflammation biomarkers in a model of abdominal sepsis in rats. Methods: Twelve Wistar rats were randomly assigned to two groups of 6 rats each. Under anesthesia, abdominal sepsis was induced by cecal ligation and puncture (CLP). Rats were observed for 24 hours. Group 1: sepsis; group 2: sepsis + pneumoperitoneum with CO2 for 30 min. Six hours after pneumoperitoneum deflation, blood samples were collected by cardiac puncture to measure (TNFα), interleukin-1β (IL-1β), interleukin-6 (IL-6), C-reactive protein (PCR) and procalcitonin (PCT). Statistical analysis using Student t test (p<0.05). Results: All animals survived. The rats with sepsis + pneumoperitoneum had levels of TNFα, IL-1β and IL-6 (542.4 ± 21pg / mL; 312.1 ± 12 pg / mL and 115.4 ± 11 pg / mL (respectively ), significantly lower than in the sepsis group (p = 0.01). Serum cytokine levels in the sepsis group were TNFα = 733.2 ± 24pg / mL; IL-1β = 432.1 ± 15 pg / mL and IL-6 = 232.3 ± 16 pg / mL. Serum levels of PCT and CRP were significantly lower in the sepsis + pneumoperitoneum group than in the sepsis group. (p=0.01). Conclusion: In conclusion, pneumoperitoneum CO2 attenuates the biomarkers of the acute phase inflammatory response associated with perioperative sepsis by PLC.

Downloads

Download data is not yet available.
Published
22-05-2020
How to Cite
SILVA, C. D.; ARAÚJO SILVA, E. M.; MEDEIROS, V. B.; MEDEIROS, A. C. EFFECT OF PNEUMOPERITONEUM IN MODEL OF ABDOMINAL SEPSIS. JOURNAL OF SURGICAL AND CLINICAL RESEARCH, v. 11, n. 1, p. 31-38, 22 May 2020.
Section
ORIGINAL ARTICLE