Effect of Propolis Extract on Biodistribution of Sodium Pertechnetate (Na99mTcO4)

Abstract

Objective: Propolis is a bee product useful natural substance applied in medicine. We have investigated the effect of propolis extract on biodistribution of sodium pertechnetate (Na^99mTcO₄)and laboratorial parameters in rats. Methods: It was administered, by gavage, 1.0ml/day of propolis into 10 rats (treated group) during 10 days and 1.0 ml/day of saline solution into other 10 rats (control group), by the same via and period. On the 10^th day, after 60min, all the animals received 0.1ml of Na^99mTcO₄ by ocular via. 50% of each group of rats was killed after 10min and other 50%, after 30min. Samples of brain, stomach, small bowel, liver, kidneys, thyroid and lungs were isolated and the %ATI/g of each organ was calculated. Tissue samples were taken from all the organs and stained by HE and PAS. Data were compared by Mann–Whitney and Student’s t-tests (p<0.05). Results: There was a statistically significant increase in the %ATI/g, from control to treated group, respectively, in small bowel (0.15±0.01 to 0.32±0.09, after 10min and 0.32±0.03 to 0.42±0.02, after 30min) and blood (0.22±0.01 to 0.48±0.02, after10min and 0.38±0.02 to 0.51±0.04, after 30min).Conclusion: Propolis extract facilitated the uptake of Na^99mTcO₄ in some organs of rats and increased levels of some laboratorial parameters.

Keywords: Propolis. Herbal Medicine. Sodium Pertechnetate Tc 99m. Radiopharmaceuticals.Rats