Simvastatin Effects on Skeletal Muscle Using the Biodistribution of 99mTc Sestamibi

Authors

  • Natania Carol Cavalcante Rezende Federal University of Rio Grande do Norte
  • Sâmea Costa Pinheiro Guerra
  • Tatiane Uetti Gomes Fernandes
  • Yngra Bastos Mesquita Minora de Almeida
  • Robson Macedo
  • Ítalo Medeiros Azevedo http://orcid.org/0000-0002-1507-7401
  • Aldo Cunha Medeiros

DOI:

https://doi.org/10.20398/jscr.v5i2.7265

Keywords:

Simvastatin, Sepsis, 99mTc-sestamibi, Skeletal muscle, Biological availability

Abstract

Objective: Examining the effects of simvastatin on skeletal muscles in an experimental model of abdominal sepsis in rats through the biodistribution of 99mTc-sestamibi. Methods: Wistar rats were randomly assigned to 2 groups: with abdominal sepsis (n = 12) and without sepsis (n = 12). Six animals with sepsis and 6 controls were injected with a daily dose of 5 mg/kg/day of simvastatin by gavage for 3 days before induction of peritonitis and 4 hours before surgical procedure. The others received 1ml of 0.9% saline solution orally. The animals were anesthetized with 20mg/kg of xylazine and 50mg/kg of ketamine i.p. Cecal ligation and puncture were performed by laparotomy. The rats were under observation for 24 hours and their survival time was recorded. The animals were re-anesthetized and 0.1 ml of 99mTc-sestamibi was administered by i.v. 30 minutes later,the thigh muscle was biopsied for percentage of radioactivity per gram of tissue (ATI%/g) determination, measured by the automatic gamma counter Wizard Counter, PerkinElmer,Finland. Results: ATI%/g of Tc99m-sestamibi was higher in muscle samples of groups treated with simvastatin sepsis ((1.82?0.21), compared with saline-treated (1.07?0.19) and control groups (1.18?0.31;1.26?0.24); this is a statistically significant difference (p<0.001). Conclusion: The data resulting from this study allows for concluding that the pre-treatment with simvastatin contributed to a biodistribution increase of 99mTc-sestamibi into the skeletal muscle in the abdominal sepsis model in rats.

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Published

03-06-2015

How to Cite

REZENDE, N. C. C.; GUERRA, S. C. P.; FERNANDES, T. U. G.; ALMEIDA, Y. B. M. M. de; MACEDO, R.; AZEVEDO, Ítalo M.; MEDEIROS, A. C. Simvastatin Effects on Skeletal Muscle Using the Biodistribution of 99mTc Sestamibi. JOURNAL OF SURGICAL AND CLINICAL RESEARCH, [S. l.], v. 5, n. 2, p. 92–99, 2015. DOI: 10.20398/jscr.v5i2.7265. Disponível em: https://periodicos.ufrn.br/jscr/article/view/7265. Acesso em: 24 sep. 2022.

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Section

ORIGINAL ARTICLE