Effect of Ringer's solution submitted to magnetic field in renal ischemia-reperfusion model in rats
Purpose: To evaluate the effect of hydration with simple Ringer's solution submitted to magnetic field in rats under renal ischemia and reperfusion (I/R) model, using biochemical and histopathological evaluation. Methods: Wistar rats were allocated into 2 equal groups of 6 animals each: Renal ischemia/reperfusion group + hydration with magnetic Ringer (mag Ringer). The left kidney underwent ischemia for 30 minutes. The right kidney was not manipulated. Thereafter, i.v. hydration was performed with 3 ml mag Ringer; Group renal I/R + hidration with simple Ringer. The simple Ringer solution was submitted to a magnetic field of 20mT intensity for 2 hours immediately prior to the surgical procedure. After 24 hours, under anesthesia blood was collected for dosing serum urea and creatinine. Histopathological analysis was performed on the left kidney (HE staining). Results: There was no significant difference between the creatinine (p=0.764) and urea (p = 0.926) values between the control and test groups (non-parametric Mann-Whitney test). Comparing the histopathological scores, there was a significant difference between the groups, with higher levels of lesions in the mag Ringer treated group (p=0.025) - Mann-Whitney test. Conclusion: The use of Ringer's solution submitted to magnetic field for intravenous hydration in a renal ischemia/reperfusion model did not alter the renal function and had a negative influence on the histopathological findings on the kidney, when compared to control.
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